To support advanced single-cell research and translational medicine, SKAN has established a state-of-the-art Flow Cytometry Facility equipped with two world-class platforms: the BD LSRFortessa™ X-20 Multi-Color Flow Cytometer for high-dimensional cellular analysis and the BD FACSAria™ Fusion High-Speed Cell Sorter for precise isolation of rare and functionally important cell populations. Together, these instruments provide comprehensive capabilities for both cellular characterization and cell sorting, enabling cutting-edge research in stem cell biology, immunology, ageing, regenerative medicine, oncology, and precision therapeutics.
BD LSRFortessa™ X-20 Multi-Color Flow Cytometer
The BD LSRFortessa™ X-20 is a state-of-the-art analytical flow cytometer that includes five excitation lasers (355 nm UV, 405 nm Violet, 488 nm Blue, 561 nm Yellow-Green, and 640 nm Red) and 18 fluorescence detection channels in addition to forward and side scatter parameters. With this configuration, we can measure up to 18 cellular markers simultaneously and perform advanced immunophenotyping, stem cell characterization, intracellular signaling and biomarker discovery.
The instrument is optimized for sensitive detection of rare cell populations and allows for the development of sophisticated multi-colour panels for comprehensive cellular profiling. It offers fast and accurate analysis for large-scale research studies with acquisition rates up to 40,000 events per second.
BD FACSAria™ Fusion High-Speed Cell Sorter
The BD FACSAria™ Fusion is a high-end, high-speed cell sorter equipped with five built-in lasers (355 nm UV, 405 nm Violet, 488 nm Blue, 561 nm Yellow-Green, and 633 nm Red) enabling the analysis of up to 20 parameters (including 18 fluorescence channels). This system enables accurate identification and purification of rare and functionally distinct cell populations with post-sort purities of >98%.
The sorter enables two-way and four-way sorting configurations, automated single-cell deposition into 6-, 24-, 48-, 96- and 384-well plates, and temperature-controlled sample handling. The high-speed sorting (up to 70,000 events per second) enables efficient recovery of viable cells for downstream applications, such as cell culture, genomic analysis, transcriptomics, functional assays, stem cell expansion and regenerative medicine studies.